Fusion of yeast protoplasts.
نویسندگان
چکیده
منابع مشابه
Surface structure of yeast protoplasts.
The fine structure of the yeast cell wall during protoplast formation was studied by means of phase-contrast microscopy and the freeze-etching technique. The freeze-etching results indicated that at least in some cases the entire wall substance was not removed from the surface of the protoplasts. After a treatment of 30 min to 3 hr with 2% snail enzymes, an innermost thin wall layer as well as ...
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Protoplasts were isolated from five species of Gossypium. · Protoplast yield and viability were affected by incubation conditions, osmolarity, purification procedures, and cell source. Using an optimized procedure, highly viable protoplasts were isolated from cell suspensions, callus cultures, and leaf tissue of G. hirsutum, G. arboreum, G. k/otzschianum, G. harlmessii, and G. herbaceum. Protop...
متن کاملFusion of plant protoplasts by electric fields.
The electrical fusion technique of Zimmermann and Scheurich (1981 Planta 151: 26-32) has been used to fuse mesophyll protoplasts of Avena, Zea, Vigna, Petunia, and Amaranthus. Electrical fusion proves to be a simple, effective, and general fusion technique that can be controlled to form either dikaryons or large multinucleate fusion bodies. In addition, we show that Vigna mesophyll protoplasts ...
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Novozym 234 (Novo lndustri A/S, Bagsraerd, Denmark) is a comparatively cheap and highly effective commercial preparation, derived from the fungus 7richoderma harziunum, which is used for the enzymic degradation of the cell wall in many yeasts and filamentous fungi in order to release protoplasts [ 1 , 21. The factors involved in protoplast isolation using this system have been investigated by C...
متن کاملLaser-induced fusion of mammalian cells and plant protoplasts.
An ultraviolet-laser microbeam was shown to be suitable for inducing fusion of individually selected plant protoplasts or of B-lymphocytes with myeloma cells. The fusion took place in normal culture medium and the fusogenic condition perturbed the cells only for a fraction of a millisecond. Without manipulating the cell culture except for exposing the cells to laser light, fusion products betwe...
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ژورنال
عنوان ژورنال: Agricultural and Biological Chemistry
سال: 1977
ISSN: 0002-1369,1881-1280
DOI: 10.1271/bbb1961.41.1829